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STREAKING FOR ISOLATION

From the 1993 Woodrow Wilson Biology Institute


TO STREAK A NUTRIENT AGAR
PLATE FOR ISOLATION OF MICROBES:

  1. Mark the bottom of the Petri dish into thirds. Label the plate with your name, date, and the name of the organism to be streaked.




  2. Turn plate over.

  3. Flame inoculating loop. Touch loop to surface of agar to cool. Touch bacterial colony (or inoculated broth) and streak the sterile plate as illustrated below. Be careful to hold lid over plate to minimize contamination.




  4. Rotate plate 90deg.. Flame loop. Touch edge of agar to cool loop. Streak by drawing sterilized loop at a right angle through previous streak as illustrated below. You should go through first streak only once to pick up the fewest microbes possible.




  5. Rotate plate 90deg.. Flame loop. Touch edge of agar to cool loop. Streak by drawing sterilized loop at a right angle through second streak as illustrated below. You should go through second streak only once to pick up the fewest microbes possible.




  6. Replace lid and invert plate for incubation at proper temperature.

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