Transformation of E.COLI

Objective

Students investigate one mechanism of genetic exchange through insertion of plasmid pBR322 DNA, which carries the gene for antibiotic resistance to ampicillin and tetracycline, into competent wild-type E. coli cells.

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• Background
  
• Pre-Laboratory Preperation
  
• Materials
  
• Method
  
• Analysis
  
• Kit Materials
  

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