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Materials

(per team of four)

115 mL capped culture tube containing culture of E. coli in nutrient broth (4-6 hrs.)
10 mL calcium chloride
20 mL (10%) Chloroxreg. solution dispensed in a 50 mL beaker
4 Petri dishes, 2 with plain nutrient agar (marked "P"), 2 with tetracycline nutrient agar (marked "T")
2 5 mL pipets
3 1 mL pipets
2 Sterile cotton swab applicators
2 Microfuge tubes
1 Centrifuge tube with cap, 15 mL, 120 mm (sterile)
4 Safety goggles
4 "Student Study Sheets" copymaster
4 "Student Analysis Sheets" copymaster
(Per class)
2Centrifuges
2 Incubators or ovens
1 Ice bath and warm water bath (37oC)
1 Biosafety bag

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Copyright © 1996
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