| 1 | 15 mL capped culture tube containing culture of E. coli in nutrient broth (4-6 hrs.) |
|
10 | mL calcium chloride |
|
20 | mL (10%) Chloroxreg. solution dispensed in a 50 mL beaker |
|
4 | Petri dishes, 2 with plain nutrient agar (marked "P"),
2 with tetracycline nutrient agar (marked "T") |
|
2 | 5 mL pipets |
|
3 | 1 mL pipets |
|
2 | Sterile cotton swab applicators |
|
2 | Microfuge tubes |
|
1 | Centrifuge tube with cap, 15 mL, 120 mm (sterile) |
|
4 | Safety goggles |
|
4 | "Student Study Sheets" copymaster |
|
4 | "Student Analysis Sheets" copymaster |
| (Per class) |
| 2 | Centrifuges |
|
2 | Incubators or ovens |
|
1 | Ice bath and warm water bath (37oC) |
|
1 | Biosafety bag
|