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Techniques Lab A: Manipulating Small Volumes


Date:__________________ Period:____________

Activity Sheet

  1. Complete the following conversions:
    a. 1 µL= ________ mL             d. _______ µL = 1.5 mL
    b. 100 µL= _______ mL e. _______ µL = 0.06 mL
    c. 250 µL= _______ mL f. _______ µL = 0.003 mL

  2. Put the following volumes in order from largest to smallest.

    1. 2.5 mL, 250 µL, 0.025 mL, 2.5 µL: __________, __________, __________, __________.

    2. 100 µL, 0.01 mL, 250 µL, 0.015 mL: __________, __________, __________, __________.

  3. Explain the reason for each of the following rules:

    1. Always set the micropipet within its designated range.

    2. Always use a micropipet with a tip.

    3. Always hold a loaded micropipet in a vertical position.

    4. Always release the micropipet plunger slowly.

    5. Observe the volume of liquid that is measured by micropipets a, b, and c.

    a. 550 µL                                           b. 15 µL                                          c. 35 µL

    Which micropipet (a, b, or c) is the P-20? ________    What is its range? _____________
    Which micropipet (a, b, or c) is the P-200? ________    What is its range? _____________
    Which micropipet (a, b, or c) is the P-1000? ________    What is its range? _____________

  4. Select the appropriate micropipet and show what the dial should read to measure each of the following amounts of liquid. Write the amount on the line beneath the correct drawing.

    a. 150 µL     b. 2.5 µL     c. 300 µL     d. 7 µL


  5. Why is it important to balance a centrifuge before turning it on?

  6. Show how you would arrange the given number of tubes in each centrifuge to balance the load. If you decide that you must add or remove tubes, explain.

  7. What is the approximate volume of a reaction tube? ____________________

    (HINT: How could you determine the volume? Do so with solution V...)

    POST LAB: Check with your teacher.

Background       Part 1       Part 2       Activity       Teacher's Guide

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