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Mammalian DNA Extraction: Teacher's Notes

I originally received this lab from Steve Clark, Monterey High School, Monterey, Calif. and have used it for several years with some modifications I made. I use this lab as an introduction to DNA science to demonstrate one method of obtaining DNA from cells. This can be done with dog testes or thymus glands. It is much more memorable for the students if you use dog testes (from you local vet--Call ahead and ask him to freeze his next neuter). Keep them frozen until you use them. You can use them straight from the freezer. This lab can be done in a 40 minute span of time with excellent results. Be sure you do not use the ends of the testes. Most of the DNA is in the center.


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