-Advertisement-
  About AE   About NHM   Contact Us   Terms of Use   Copyright Info   Privacy Policy   Advertising Policies   Site Map
   
Custom Search of AE Site
spacer spacer

Modeling the Mitochondrial DNA Clock Work Sheet:
Mitochondrial DNA

In the spaces below record the series of random point mutations determined by the selection of the site and base change tiles. On the accompanying sheet, also record the changes in mtDNA sequence for your "population" as the tiles are chosen. When all the rounds of mutation have been completed, you will collect the altered sequences for all of our "populations". Your homework is to examine the patterns of alteration between the chains of mtDNA in the separated populations in detail and to relate your observations to the timing of separation during this activity.

Mutation 1
Population 1: site # ___becomes ____All others: site # ___becomes ____

Mutation 2
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
All others: site # ___becomes ____

Mutation 3
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
Population 3: site # ___becomes ____All others: site # ___becomes ____

Mutation 4
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
Population 3: site # ___becomes ____Population 4: site # ___becomes ____
All others: site # ___becomes ____

Mutation 5
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
Population 3: site # ___becomes ____Population 4: site # ___becomes ____
Population 5: site # ___becomes ____Population 6: site # ___becomes ____
All others: site # ___becomes ____

Mutation 6
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
Population 3: site # ___becomes ____Population 4: site # ___becomes ____
Population 5: site # ___becomes ____Population 6: site # ___becomes ____
Population 7: site # ___becomes ____Population 8: site # ___becomes ____
All others: site # ___becomes ____

Mutation 7
Population 1: site # ___becomes ____Population 2: site # ___becomes ____
Population 3: site # ___becomes ____Population 4: site # ___becomes ____
Population 5: site # ___becomes ____Population 6: site # ___becomes ____
Population 7: site # ___becomes ____Population 8: site # ___becomes ____
Population 9: site # ___becomes ____Population 10: site # ___becomes ____

Mutation 8
Population 1: site # ___becomes ____Population 2: site # ____becomes ____
Population 3: site # ___becomes ____Population 4: site # ___becomes ____
Population 5: site # ___becomes ____Population 6: site # ___becomes ____
Population 7: site # ___becomes ____Population 8: site # ___becomes ____
Population 9: site # ___becomes ____Population 10: site # ___becomes ____
Original mtDNA strand:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30



As the mutations are called out for your population, circle each base site that is mutated and indicate the base to which it changed below the site number. When all mutation steps have been accomplished, write out your new, altered mtDNA sequence in the spaces below:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population number: _____

Before moving to the analysis of altered patterns in all of the ten "populations" this activity produced, write for five minutes, in the space below, a detailed list of the observations and impressions you made during the "mutation" process.










Below, record the new base sequence of each of the ten diverged populations developed in this activity.

Population 1:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 2:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 3:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 4:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 5:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 6:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 7:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 8:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 9:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Population 10:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

For homework address the following questions in detail. Be prepared to discuss them in class.

  1. Record a detailed list of all the observations and comparisons you can make about each of these new "strands" of mtDNA.

  2. What relationships can you infer between the "strand" changes and the pattern of reproductive isolation imposed on the groups during this activity?

Student Introduction | Teacher Background




Activities-To-Go Index


Activities Exchange Index


 
Custom Search on the AE Site

 

-Advertisement-