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ONION DNA EXTRACTION
graduated cylinders (10ml and 100ml)
15-ml test tube
test tube rack or 250 ml beaker
glass stirring rod
Adolph's natural meat tenderizer
ice cold 95% ethanol
20 ml detergent
20 g non-iodized salt
180 ml distilled water
5% meat tenderizer solution:
5 g meat tenderizer
95 ml distilled water
Cut an inch square out of the center of 3 medium onions. Chop and place in a blender.
Add 100 ml of detergent/salt solution.
Blend on high 30 sec-1 minute.
Strain the mixture into a beaker using a strainer with a coffee filter.
Add 20-30 ml meat tenderizer and stir to mix.
Place 6 ml filtrate in a test tube.
Pour 6 ml ice cold ethanol carefully down the side of the tube to form a layer.
Let the mixture sit undisturbed 2-3 minutes until bubbling stops.
The DNA will float in the alcohol. Swirl a glass stirring rod at the interface of the two layers to see the small threads of DNA.
Modified from: "Isolation of DNA from Onion" Ellen Averill
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