THYMUS DNA EXTRACTIONS
Lana Hays
Materials
fresh thymus
blender
beaker
sugar
pipet
centrifuge tube with cap
Bufferin (325mg)
knife
graduated cylinders (10ml,100ml) |
epsom salts
distilled water
centrifuge
95% ice cold ethanol
15 ml test tubetest tube rack or beaker
Palmolive detergent
non-iodized salt
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Solutions
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- prep buffer solution:
- 57 g granulated sugar
- 1 buffered aspirin
- 3 g epsom salts
- add distilled water for a total of 500 ml
- 10% detergent solution:
- 90 ml distilled water
- 10 ml Palmolive detergent
- salt solution:
- 29.2 g non-iodized salt
- add distilled water for a total volume of 250 ml
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Protocol
- Cut out a chunk of liver or thymus 1 inch square and place in the blender.
- Add 100 -150 ml prep buffer and 10 ml detergent solution to the blender.
- Blend for 1 minute or until the mixture is smooth.
- Pour the mixture into a beaker.
- Transfer 1 ml of the mixture to a centrifuge tube.
- Add 2 ml of salt solution, cap, and shake for 2 minutes.
- Centrifuge for 7 minutes in a balanced centrifuge.
- Carefully remove the tube from the centrifuge and note the two layers:
lower layer - pellet
*upper layer - liquid (supernatant) and what has the DNA in it.
- Pipet or carefully pour the liquid into a clean test tube.
- Pour 5 ml ice cold ethanol carefully down the side of the tube to form a layer.
- Let the mixture sit undisturbed for a minute or two.
- The DNA will float in the alcohol. The DNA of the thymus will be long threads that easily spool.
Modified from:
"Generic, All Purpose DNA Extraction from Meat Protocol" Judy Brown
"Mammalian DNA Extraction" Theresa Knapp
Onion |
Wheat Germ |
Lima Bean Bacteria |
Thymus |
Yeast |
Data Sheet and Questions |
Design Your Own DNA Extraction Protocol |
Teacher Information | References
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Activities-To-Go Index
