Materials

Solutions

Protocol

1. Add 100 ml distilled water to a beaker and heat to 50-60^oC.
2. Add 1.5 g wheat germ and mix until dissolved.
3. Add 5 ml detergent. Maintain 50-60^oC temperature and stir for 5 minutes.
4. Add 3 g meat tenderizer.
5. Add baking soda solution to bring the pH to approximately 8.0.
6. Maintain the 50-60^oC temperature and stir for 10 minutes.
7. Remove from heat.
8. Add 6 ml of the solution to a test tube and cool to room temperature.
9. Pour 6 ml ice cold ethanol carefully down the side of the tube to form a layer.
10. Let the mixture sit undisturbed 2-3 minutes until bubbling stops.
11. The DNA will float in the alcohol. Swirl a glass stirring rod at the interface of the two layers to see the small threads of DNA.

Modified from: "Wheat Germ DNA Extraction" Judy Brown

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