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Bertino.DNA extraction simplified '95
modified from L. Hays and A. Bertino, WWNFF, '93

The simplified plan for DNA extraction

Original directions

  1. 100 ml of water between 50-60°C.
  2. Add 1.5 grams raw wheat germ & stir
  3. Add 5 ml of detergent
  4. Add 3 grams Adolph's Meat Tenderizer
  5. Adjust pH to 8 using baking soda
  6. Maintain temp (50-60° C) & stir for 10 min.
  7. Remove from heat, pour 6 ml into test tube
  8. Pour 6 ml of ice cold ethanol slowly down the inside edge of the test tube on top of the suspension
  9. Wait 5 minutes and observe
  10. Remove the DNA by spooling it.
  11. place DNA 'glob' on preweighed filter paper and allow to dry. Reweigh. Determine % yield of DNA

Today's plan

  1. To 1/2 cup hot water, add
  2. 1 level tsp. wheat germ & stir
  3. Add test tube of detergent
  4. Dump in a level tsp of Adolph's
  5. Forget the baking soda
  6. Stir for a couple more minutes
  7. Pour 3/4" or so in big test tube
  8. Pour t.t. of ethanol slowly down the inside edge of the big t.t. on top of the suspension
  9. Take a look! DNA strands will start to form immediately
  10. Spool the DNA on the wooden skewer provided
  11. Do it home......

A napkin has been provided to help clean up your mess. The zip-lock bag has been provided so that you might either keep what you like as a reminder or disgard neatly into the trash.

For further information contact:
Anthony J. Bertino
Canandaigua Academy
1 Academy Circle
Canandaigua, NY 14424

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