BLOOD TYPING LAB
Shared Materials:
-
Simulated Blood Samples
- Bennett
- Batey
- Robertson
- Parris
- Bain
- Morris
- Unknown crime scene blood samples
- Anti-A Simulated Typing Serum
- Anti-B Simulated Typing Serum
- Anti-Rh Simulated Typing Serum
Objective:
Each team will determine and compare the blood type for known and unknown samples of blood.
Identify three suspects whose blood was found at the crime scene
Procedure:
- Pre-label each of your six blood typing slides as follows:
- Slide #1: Bennett
- Slide #2: Batey
- Slide #3: Robertson
- Slide #4: Parris
- Slide #5: Bain
- Slide #6: Morris
- Place two drops of each known blood type in a separate well-marked A,B and Rh on each
slide.
- Add 2 drops of the simulated anti-A serum in each A well on the slides.
- Add 2 drops of the simulated anti-B serum in each B well on the slides.
- Add 2 drops of the simulated anti-Rh serum in each Rh well on the slides.
- Use separate toothpicks to stir each sample of serum and blood. Record your observations and
results on data table 1. Repeat the procedure for the unknown crime samples. Record on table 2.
| DATA TABLE 1: Agglutination Reactions |
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Anti-A Serum
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Anti-B Serum
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Anti-Rh Serum
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Blood Type
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Observations
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Slide #1:
Bennett
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Slide #2:
Batey
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Slide #3:
Robertson
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Slide #4:
Parris
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Slide #5:
Bain
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Slide #6:
Morris
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A POSITIVE TEST IS INDICATED BY A STRONG AGGLUTINATION REACTION.
| Data Table 2
Agglutination Reactions: Unknown Samples |
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Anti-A Serum
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Anti-B Serum
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Anti-Rh Serum
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Blood Type
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Observations
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|
Sample # 1
Sample # 2
Sample # 3
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ANALYSIS:
Determine the origin of the unknown blood types found at the crime scene. Record your
observations .
SAMPLE # 1 matches with the known blood sample of _______________________.
SAMPLE # 2 matches with the known blood sample of _______________________.
SAMPLE # 3 matches with the known blood sample of _______________________.
AGGLUTINATION REACTIONS OF ABO BLOOD-TYPING SERA
|
ANTI-A SERUM
agglutination
no agglutination
agglutination
no agglutination
|
ANTI-B SERUM
no agglutination
agglutination
agglutination
no agglutination
|
Blood Type
Type A
Type B
Type AB
Type O
|
FIBER LAB
Identify notable characteristics of blood stained fibers removed from the crime scene. Using a microscope,
sketch the weave pattern identifiying each as twill, satin or knitted.
Sample 1
Identification of pattern:
|
Drawing:
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Sample 2:
Identification of pattern:
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Drawing:
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Sample 3:
Identification of pattern:
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Drawing:
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Sample 1 belongs to:_____________
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Sample 2 belongs to:_____________
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Sample 3 belongs to:_____________
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Index
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