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BLOOD TYPING LAB



Shared Materials:

  1. Simulated Blood Samples
    1. Bennett
    2. Batey
    3. Robertson
    4. Parris
    5. Bain
    6. Morris
  2. Unknown crime scene blood samples

    • Anti-A Simulated Typing Serum
    • Anti-B Simulated Typing Serum
    • Anti-Rh Simulated Typing Serum



Objective:

Each team will determine and compare the blood type for known and unknown samples of blood. Identify three suspects whose blood was found at the crime scene



Procedure:

  1. Pre-label each of your six blood typing slides as follows:

    • Slide #1: Bennett
    • Slide #2: Batey
    • Slide #3: Robertson
    • Slide #4: Parris
    • Slide #5: Bain
    • Slide #6: Morris


  2. Place two drops of each known blood type in a separate well-marked A,B and Rh on each slide.
    1. Add 2 drops of the simulated anti-A serum in each A well on the slides.
    2. Add 2 drops of the simulated anti-B serum in each B well on the slides.
    3. Add 2 drops of the simulated anti-Rh serum in each Rh well on the slides.


  3. Use separate toothpicks to stir each sample of serum and blood. Record your observations and results on data table 1. Repeat the procedure for the unknown crime samples. Record on table 2.


DATA TABLE 1: Agglutination Reactions
Anti-A Serum Anti-B Serum Anti-Rh Serum Blood Type Observations

Slide #1:
Bennett

Slide #2:
Batey

Slide #3:
Robertson

Slide #4:
Parris

Slide #5:
Bain

Slide #6:
Morris



A POSITIVE TEST IS INDICATED BY A STRONG AGGLUTINATION REACTION.



Data Table 2 Agglutination Reactions: Unknown Samples

Anti-A Serum

Anti-B Serum

Anti-Rh Serum

Blood Type

Observations

Sample # 1


Sample # 2


Sample # 3



ANALYSIS:

Determine the origin of the unknown blood types found at the crime scene. Record your observations .

SAMPLE # 1 matches with the known blood sample of _______________________.

SAMPLE # 2 matches with the known blood sample of _______________________.

SAMPLE # 3 matches with the known blood sample of _______________________.



AGGLUTINATION REACTIONS OF ABO BLOOD-TYPING SERA

ANTI-A SERUM


agglutination

no agglutination

agglutination

no agglutination

ANTI-B SERUM


no agglutination

agglutination

agglutination

no agglutination

Blood Type


Type A

Type B

Type AB

Type O



FIBER LAB

Identify notable characteristics of blood stained fibers removed from the crime scene. Using a microscope, sketch the weave pattern identifiying each as twill, satin or knitted.


Sample 1 Identification of pattern:


Drawing:


Sample 2: Identification of pattern:


Drawing:


Sample 3: Identification of pattern:


Drawing:



Sample 1 belongs to:_____________ Sample 2 belongs to:_____________ Sample 3 belongs to:_____________


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