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CBL Interfacing

Photosynthesis In Isolated Chloroplasts


Extensions

In this investigation we learned an approach for measuring the rate of photosynthesis. Using the technique which you applied, design an experiment to determine the effect of two factors on the rate of the reaction:

A. Light Intensity
B. Wavelength (color)

The investigation will contain the following components:

  1. Hypothesis
  2. General Experimental Design
  3. Materials
  4. Safety Considerations
  5. Procedure
  6. Result
  7. Analysis of Data
  8. Conclusion

Steps 1-5 must be done ahead of time. Your teacher will assign you to submit your proposal ahead of time, correct it and then allow you to carry out your experiment.

Hypothesis
State your hypothesis in an if....then...format. The hypothesis is an "educated guess" so state why you expect the results which you have predicted. State the hypothesis in a form that includes some specifics. If you do, the hypothesis should guide you through the general design of your experiment.

General Experimental Design
What is your independent variable? How will you set up your controls to insure that you have one and only one independent variable? What is your dependent variable and how will you measure it?

Materials
Make a list of all materials which you will need to conduct your experiment. This takes careful, careful planning.

Safety Considerations
Are you working with chemicals, acids, bases? Are you heating any solutions? How will you protect you and your classmates during your experiment?

Procedure
Determine a reasonable, logical order for carrying out your experiment. Write out a step by step procedure. Include all details.

Results
Prepare a data sheet with tables and a set of axes for a graph. Be sure to enter all data, including any unexpected observations.

Analysis of Data
Determine what manipulations of data will have to be carried out to make an analysis of your results most meaningful. Would a graph make it easier to analyze your results?

Conclusion
What do you conclude based on the analysis of data? Does the data support or refute your hypothesis? Explain.


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