CBL Interfacing
Photosynthesis In Isolated Chloroplasts
Processing The Data
J.
Entering Your Data on Calculator Data Tables
A menu appears. Choose QUIT.
Press STAT.
A menu appears. Choose edit.
Arrow up to highlight L1.
Press CLEAR and then ENTER.
L1 is now clear. Enter the times of the absorbance readings performing
the following steps.
Type 0, ENTER. Type 5, ENTER. Type 10, ENTER. Type 15, ENTER.
Now arrow right and up to highlight L2.
Press CLEAR and then ENTER.
L2 is now clear. Enter the absorbances of cuvette 2 using the
same procedure as entering the times. (i.e. Type in the absorbance
at 0, 5, 10 and 15 minutes and press ENTER after each entry.)
Repeat the above procedure to enter your data for cuvette 3 onto
L3 and cuvette 4 onto L4.
K.
Setting Up Your Plots
Cuvette 2, Dark
Press 2nd, STAT PLOT.
A menu appears. Choose Plot 1.
The screen shows Plot 1. The following selections should be highlighted
by moving the cursor to the appropriate item and pressing enter.
On
Type: line graph
X lists: L1
Y lists: L2
Mark: .
Cuvette 3, Light, Unboiled
Press 2nd, STAT PLOT.
A menu appears. Choose Plot 2.
The screen shows Plot 2. The following selections should be highlighted
by moving the cursor to the appropriate item and pressing enter.
On
Type: line graph
X lists: L1
Y lists: L3
Mark: .
Cuvette 4, Light, Boiled
Press 2nd, STAT PLOT.
A menu appears. Choose Plot 3.
The screen shows Plot 3. The following selections should be highlighted
by moving the cursor to the appropriate item and pressing enter.
On
Type: line graph
X lists: L1
Y lists: L4
Mark: .
Press GRAPH, ZOOM, 9. The graphs for the three cuvettes will now
appear on your screen. Press TRACE. A Blinking cursor will appear
on plot 1. By arrowing right you can move along each plot. By
arrowing down, you will move the cursor to the next plot. The
number of each plot, P1, P2 and P3 appear in the upper right hand
corner of the screen to help you keep track of which plot your
are tracing.
Just a reminder, Plot 1 is for the cuvette kept in the dark, Plot
2 is for the cuvette with the unboiled chloroplasts kept in the
light and Plot 3 is for the cuvette with the boiled chloroplasts
kept in the light.
L.
Making A Hard Copy of Your Data
The plots obtained in the experiment represents the change in
absorbance with respect to time. If available, use a TI-GRAPH
LINK to print a hard copy of the graph; otherwise make a sketch
of the graph on your data sheet.
Label each the axis, set up a scale and label each plot. Use the
trace function to determine the scale on the Y-axis. The number
value appears at the bottom of the screen.
M.
Finding the Slope of Each Line
Before starting this section you need to adjust your calculator
so that the numerical value of the slope is given with enough
decimal places.
Press MODE and arrow down to float. Then arrow right to 4. Press
ENTER.
Slope of Plot 1 (Dark)
Press STAT, arrow right, and choose LinReg (ax+b)
In the next instruction
DO TYPE THE COMMA (,).
Press 2nd L1, 2nd L2 ENTER. Several equations are shown. The slope
is given as a=.
Record the slope on your data sheet.
Be sure to note if it is positive or negative.
Slope of Plot 2 (Light, Unboiled)
Press STAT, arrow right, and choose LinReg (ax+b)
In the next instruction
DO TYPE THE COMMA (,).
Press 2nd L1, 2nd L3 ENTER. Several equations are shown. The slope
is given as a=.
Record the slope on your data sheet.
Be sure to note if it is positive or negative.
Slope of Plot 3 (Light, Boiled)
Press STAT, arrow right, and choose LinReg (ax+b)
In the next instruction
DO TYPE THE COMMA (,).
Press 2nd L1, 2nd L4 ENTER. Several equations are shown. The slope
is given as a=.
Record the slope on your data sheet. Be sure to note if it is
positive or negative.
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