CBL Interfacing

Photosynthesis In Isolated Chloroplasts

Questions

1. For each sample, describe the relationship between absorbance and time? In which sample did the absorbance drop most dramatically?
2. What is the effect of darkness on the reduction of DPIP?
3. What is the effect of boiling the chloroplast on the reduction of DPIP?
4. What was the initial rate of DPIP reduction by the unboiled chloroplasts that were incubated in the light?
5. How did the rate vary during the experiment's time course?
6. There are two major products of photosynthesis-water ad organic substances such as glucose. How would the presence of DPIP affect the production of oxygen during the light reaction? How would the presence of DPIP affect the production of organic product during the dark reaction? Explain.
7. What role did another artificial electron acceptor, ferricyanide play in elucidating the process of photosynthesis?
8. If time permits, design and set up an experiment comparing how the wavelength of light would affect reduction of DPIP. Analyze the slopes and discuss your results.

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