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Computer Interfacing

Enzyme Action: Testing Catalase Activity


Objectives

In this experiment, you will:

  • use a computer and pressure sensor to measure the destruction of hydrogen peroxide by the enzyme catalase or peroxidase at various enzyme concentrations.
  • measure and compare the initial rates of reaction for this enzyme when different concentrations of enzyme react with H2O2.
  • use a computer to measure the destruction of hydrogen peroxide by the enzyme catalase or peroxidase at various temperatures.
  • measure and compare the initial rates of reaction for the enzyme at each temperature.
  • use a computer to measure the destruction of hydrogen peroxide by the enzyme catalase or peroxidase at various pH values.
  • measure and compare the initial rates of reaction for the enzyme at each pH value.

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