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Computer Interfacing

The Effect of Alcohol on Biological Membranes


Prepare the alcohol solutions

2. Obtain the following materials:

  • Place about 10 mL of methanol in a medium sized test tube. Label this tube "T1"
  • Place about 10 mL of ethanol in a medium sized test tube. Label this tube "T2"
  • Place about 10 mL of 1-propanol in a medium sized test tube. Label this tube "T3"
  • Place about 30 mL of tap water in a small beaker.

3. Prepare six methanol solutions (0%, 20%, 40%, 60%, 80%, and 100%). To do so:

  • Use the pipette and pipette pump to add water to each of five wells in the microwell plate. See Table 1 to determine the amount of water to add to each well. If your browser cannot handle tables, go here.
  • Use a different pipette to add alcohol to each of five wells in the microwell plate. See Table 1 to determine the amount of alcohol to add to each well. If your browser cannot handle tables, go here.

4. Clean the pipette used to transfer alcohol. To do this,

  • Wipe the outside clean and empty it of liquid.
  • Draw up a little ethanol into the pipette and use the liquid to rinse the inside of the pipette.
  • Discard the ethanol.

5. Prepare six ethanol solutions. To do so, repeat Step 3, substituting ethanol for methanol. Place each solution in the second row of wells, as shown in Figure 2.

6. Prepare six propanol solutions. To do so, clean your pipette and repeat Step 3, substituting 1-propanol for methanol. Place each solution in the third row of wells, as shown in Figure 2.


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