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Health: Disease transmission and control
Level 2 2.1 Evaluate the effectiveness of hand washing and explain the importance of aseptic techniques in the hospital environment.

2.2 Conduct an experiment or participate in a class activity which demonstrates at least one mode of disease transmission. Analyze results and apply the results to real diseases.

2.3 Answer the questions: "How is tuberculosis transmitted? " and "What are its symptoms? "

2.4 Grow several kinds of bacteria and fungi on the same plate. Examine, describe and explain what happens as colonies of microorganisms grow toward each other.

Level 3:
Complete Level 2 +
3.1 Demonstrate Koch's Postulates using Erwinia carotovora, the bacterium which cause soft rot of carrots. Explain how these postulates are used to identify disease causing agents.

3.2 Demonstrate an understanding of how antibiotics work and why they do not work on viruses

Level 4:
Complete Level 3 +
4.1 Describe four ways contagious diseases are transmitted. For each, give at least one example of a disease transmitted in that manner and an example of a way disease transmission by this route could be reduced.

4.2 Discuss factors which lead to emergence of antibiotic resistant bacteria.

Science: Microbiology
Level 2 2.1 Demonstrate use of appropriate sterile technique for handling bacteria. Streak plate. Grow bacteria and transfer bacteria from one plate to another

2.2 Distinguish between bacteria and fungi when they are growing on agar plates and when they are growing on liquid media.

2.3 Examine prepared slides of Mycobacterium tuberculosis the bacterium which causes TB.

2.4 Demonstrate knowledge of the requirements for bacterial growth. (Food, water, space, etc.) and use experimental methods to show how removal of any one of these elements stops bacterial growth.

2.5 Describe the growth requirements for the bacterium which causes tuberculosis.

2.6 Design and conduct an experiment to determine the source of bacteria growing in a flask. Your experimental design must have appropriate controls and a single variable.

Level 3:
Complete Level 2 +
3.1 Analyze the results of the experiment done in 2.6 above. State conclusions which can be based on your experimental results. Discuss strengths and weaknesses of your experiment.

3.2 Prepare a bacterial smear; stain and examine with oil immersion lens. Identify bacterial types (cocci,bacilli,etc.)

3.3 Design and conduct an experiment which demonstrates different nutrient requirements of Lac minus and Lac plus strains of E. coli. (Lac minus does not produce the enzyme lactase.)

Level 4:
Complete Level 3 +
4.1 Design an experiment to confirm what you learned in 2.6 and 3.1 or to answer a question raised by analysis you did in 3.1.

4.2 Distinguish between encapsulated bacteria and those which are not. Describe the significance of bacterial capsules in disease control, treatment, and prevention.

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